Sample Set Annotation Tool: Transcriptomic profiling in childhood H1N1/09 influenza reveals reduced expression of protein synthesis genes. GSE42026

Study Design
Assay
Sample Info
Files
Info
Samples
Group Sets
Analysis

Purpose

In order to understand the immunopathogenesis of severe influenza H1N1/09, we compared the whole blood RNA transcriptome of children hospitalised with H1N1/09 infection with that of children hospitalised with RSV or bacterial infection. Blood was collected into PAX gene tubes (PreAnalytiX). Total RNA integrity was assessed using an Agilent 2100 Bioanalyzer (Agilent, Palo Alto, CA). Labeled cRNA was hybridized to Illumina Human HT-12 v3 Beadchips.

Hypothesis

Experimental Design

Observational, prospective study. Patients were recruited at or after presentation to hospital. Main inclusion criteria were presence of illness of sufficient severity to warrant blood tests for clinical reasons, in child aged <17 years. Patient numbers recruited were as follows: H1N1/09 infection (n=25) RSV (n=34) (n=21), and healthy paediatric controls (n=33). After exclusion of children with co-infections, numbers of arrays analysed in were as follows: H1N1/09 n=19; RSV n=22; bacterial infection n=18; controls n=33

Experimental Variables

Controls

Methods

Additional Information

Expected Samples:

92 Samples Loaded: 92

Species

Human (Homo sapiens)

Sample Source

Whole Blood

Disease

RSV, Influenza, bacterial infection

Treatment Protocol

Growth Protocol

Extraction Protocol

Storage Conditions

Sample Set Spreadsheet

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Samples Preview

Sample ID	!Sample Title	Gender	tissue	group	Infecting pathogen
GSM1030788	WB-bact-m117	female	whole blood	Bacterial infection	gram positive bacterial infection
GSM1030789	WB-bact-m120	female	whole blood	Bacterial infection	gram positive bacterial infection
GSM1030790	WB-bact-m164	female	whole blood	Bacterial infection	gram positive bacterial infection
GSM1030791	WB-bact-m173	male	whole blood	Bacterial infection	gram positive bacterial infection
GSM1030792	WB-bact-m180	female	whole blood	Bacterial infection	gram positive bacterial infection

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File
sampleset4000025_sampleannotations_Group.csv Sample Set Spreadsheet
sampleset4000025_sampleannotations_Group.csv Sample Set Spreadsheet

Samples Viewer / Editor

All fields are editable except the "Sample ID" column. To edit a cell, click within the cell. To edit a "date" cell, click on the calendar icon. To cancel an edit, press the ESC key.

Sample ID	!Sample Title	Gender	Tissue	Group	Infecting pathogen
GSM1030788	WB-bact-m117	female	whole blood	Bacterial infection	gram positive bacterial infection
GSM1030789	WB-bact-m120	female	whole blood	Bacterial infection	gram positive bacterial infection
GSM1030790	WB-bact-m164	female	whole blood	Bacterial infection	gram positive bacterial infection
GSM1030791	WB-bact-m173	male	whole blood	Bacterial infection	gram positive bacterial infection
GSM1030792	WB-bact-m180	female	whole blood	Bacterial infection	gram positive bacterial infection
GSM1030793	WB-bact-m99	male	whole blood	Bacterial infection	gram positive bacterial infection
GSM1030794	WB-bact-m145	female	whole blood	Bacterial infection	gram positive bacterial infection
GSM1030795	WB-bact-m177	male	whole blood	Bacterial infection	gram positive bacterial infection
GSM1030796	WB-bact-m179	female	whole blood	Bacterial infection	gram positive bacterial infection
GSM1030797	WB-bact-m191	male	whole blood	Bacterial infection	gram positive bacterial infection
GSM1030798	WB-bact-m192	male	whole blood	Bacterial infection	gram positive bacterial infection
GSM1030799	WB-bact-m216	female	whole blood	Bacterial infection	gram positive bacterial infection
GSM1030800	WB-bact-m226	male	whole blood	Bacterial infection	gram positive bacterial infection
GSM1030801	WB-bact-m228	female	whole blood	Bacterial infection	gram positive bacterial infection
GSM1030802	WB-bact-m236	male	whole blood	Bacterial infection	gram positive bacterial infection
GSM1030803	WB-bact-m238	female	whole blood	Bacterial infection	gram positive bacterial infection
GSM1030804	WB-bact-m239	female	whole blood	Bacterial infection	gram positive bacterial infection
GSM1030805	WB-bact-m102	female	whole blood	Bacterial infection	gram positive bacterial infection
GSM1030806	WB-control-m9	male	whole blood	Healthy Control	none
GSM1030807	WB-control-m56	female	whole blood	Healthy Control	none
GSM1030808	WB-control-m64	male	whole blood	Healthy Control	none
GSM1030809	WB-control-m68	female	whole blood	Healthy Control	none
GSM1030810	WB-control-m79	female	whole blood	Healthy Control	none
GSM1030811	WB-control-m80	male	whole blood	Healthy Control	none
GSM1030812	WB-control-m96	male	whole blood	Healthy Control	none
GSM1030813	WB-control-m107	female	whole blood	Healthy Control	none
GSM1030814	WB-control-m143	male	whole blood	Healthy Control	none
GSM1030815	WB-control-m144	female	whole blood	Healthy Control	none
GSM1030816	WB-control-m160	male	whole blood	Healthy Control	none
GSM1030817	WB-control-m161	male	whole blood	Healthy Control	none
GSM1030818	WB-control-m162	male	whole blood	Healthy Control	none
GSM1030819	WB-control-m163	female	whole blood	Healthy Control	none
GSM1030820	WB-control-m166	female	whole blood	Healthy Control	none
GSM1030821	WB-control-m176	male	whole blood	Healthy Control	none
GSM1030822	WB-control-m183	male	whole blood	Healthy Control	none
GSM1030823	WB-control-m184	male	whole blood	Healthy Control	none
GSM1030824	WB-control-m185	male	whole blood	Healthy Control	none
GSM1030825	WB-control-m186	male	whole blood	Healthy Control	none
GSM1030826	WB-control-m190	female	whole blood	Healthy Control	none
GSM1030827	WB-control-m198	female	whole blood	Healthy Control	none
GSM1030828	WB-control-m199	female	whole blood	Healthy Control	none
GSM1030829	WB-control-m200	female	whole blood	Healthy Control	none
GSM1030830	WB-control-m201	female	whole blood	Healthy Control	none
GSM1030831	WB-control-m207	female	whole blood	Healthy Control	none
GSM1030832	WB-control-m211	female	whole blood	Healthy Control	none
GSM1030833	WB-control-m212	female	whole blood	Healthy Control	none
GSM1030834	WB-control-m219	male	whole blood	Healthy Control	none
GSM1030835	WB-control-m222	male	whole blood	Healthy Control	none
GSM1030836	WB-control-m223	male	whole blood	Healthy Control	none
GSM1030837	WB-control-m231	male	whole blood	Healthy Control	none
GSM1030838	WB-control-m234	male	whole blood	Healthy Control	none
GSM1030839	WB-H1N1-m1	male	whole blood	Influenza A H1N1/09	Influenza A H1N1/09
GSM1030840	WB-H1N1-m2	male	whole blood	Influenza A H1N1/09	Influenza A H1N1/09
GSM1030841	WB-H1N1-m28	female	whole blood	Influenza A H1N1/09	Influenza A H1N1/09
GSM1030842	WB-H1N1-m37	male	whole blood	Influenza A H1N1/09	Influenza A H1N1/09
GSM1030843	WB-H1N1-m39	male	whole blood	Influenza A H1N1/09	Influenza A H1N1/09
GSM1030844	WB-H1N1-m48	female	whole blood	Influenza A H1N1/09	Influenza A H1N1/09
GSM1030845	WB-H1N1-m51	male	whole blood	Influenza A H1N1/09	Influenza A H1N1/09
GSM1030846	WB-H1N1-m57	female	whole blood	Influenza A H1N1/09	Influenza A H1N1/09
GSM1030847	WB-H1N1-m60	female	whole blood	Influenza A H1N1/09	Influenza A H1N1/09
GSM1030848	WB-H1N1-m61	female	whole blood	Influenza A H1N1/09	Influenza A H1N1/09
GSM1030849	WB-H1N1-m63	male	whole blood	Influenza A H1N1/09	Influenza A H1N1/09
GSM1030850	WB-H1N1-m72	male	whole blood	Influenza A H1N1/09	Influenza A H1N1/09
GSM1030851	WB-H1N1-m73	male	whole blood	Influenza A H1N1/09	Influenza A H1N1/09
GSM1030852	WB-H1N1-m94	male	whole blood	Influenza A H1N1/09	Influenza A H1N1/09
GSM1030853	WB-H1N1-m95	male	whole blood	Influenza A H1N1/09	Influenza A H1N1/09
GSM1030854	WB-H1N1-m138	female	whole blood	Influenza A H1N1/09	Influenza A H1N1/09
GSM1030855	WB-H1N1-m155	female	whole blood	Influenza A H1N1/09	Influenza A H1N1/09
GSM1030856	WB-H1N1-m208	female	whole blood	Influenza A H1N1/09	Influenza A H1N1/09
GSM1030857	WB-H1N1-m12	male	whole blood	Influenza A H1N1/09	Influenza A H1N1/09
GSM1030858	WB-RSV-m19	male	whole blood	RSV	RSV
GSM1030859	WB-RSV-m34	female	whole blood	RSV	RSV
GSM1030860	WB-RSV-m69	male	whole blood	RSV	RSV
GSM1030861	WB-RSV-m70	male	whole blood	RSV	RSV
GSM1030862	WB-RSV-m77	male	whole blood	RSV	RSV
GSM1030863	WB-RSV-m78	female	whole blood	RSV	RSV
GSM1030864	WB-RSV-m92	male	whole blood	RSV	RSV
GSM1030865	WB-RSV-m128	male	whole blood	RSV	RSV
GSM1030866	WB-RSV-m129	female	whole blood	RSV	RSV
GSM1030867	WB-RSV-m135	female	whole blood	RSV	RSV
GSM1030868	WB-RSV-m137	male	whole blood	RSV	RSV
GSM1030869	WB-RSV-m140	male	whole blood	RSV	RSV
GSM1030870	WB-RSV-m142	male	whole blood	RSV	RSV
GSM1030871	WB-RSV-m147	female	whole blood	RSV	RSV
GSM1030872	WB-RSV-m150	female	whole blood	RSV	RSV
GSM1030873	WB-RSV-m153	male	whole blood	RSV	RSV
GSM1030874	WB-RSV-m158	male	whole blood	RSV	RSV
GSM1030875	WB-RSV-m189	male	whole blood	RSV	RSV
GSM1030876	WB-RSV-m214	male	whole blood	RSV	RSV
GSM1030877	WB-RSV-m220	male	whole blood	RSV	RSV
GSM1030878	WB-RSV-m224	male	whole blood	RSV	RSV
GSM1030879	WB-RSV-m252	female	whole blood	RSV	RSV

Group Sets View in Gene Expression Browser

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