Sample Set Annotation Tool: Time Course of Young Adults Vaccinated with Influenza LAIV Vaccine during 2008/09 Flu Season. GSE29615

Study Design
Assay
Sample Info
Files
Info
Samples
Group Sets
Analysis

Purpose

Systems vaccinology has emerged as an interdisciplinary field that combines systems wide measurements and network and predictive modeling applied to vaccinology.Here we used the systems vaccinology approach to study the molecular mechanisms underlying the innate responses to the trivalent inactivated influenza (TIV) and live attenuated influenza (LAIV) vaccination in humans, and to identify early gene signatures that predict the magnitude of the antibody responses to influenza vaccination.

Hypothesis

Experimental Design

During the 2008 influenza season, healthy adults were vaccinated with LAIV (28 vaccinees), and blood samples isolated at days 0, 3, 7 post-vaccination. Microarrays were performed using total RNA extracted from the peripheral blood mononuclear cells of vaccinees.

Experimental Variables

Controls

Methods

Additional Information

Expected Samples:

83 Samples Loaded: 83

Species

Human (Homo sapiens)

Sample Source

PBMC

Disease

Influenza vaccine

Treatment Protocol

Growth Protocol

Extraction Protocol

Storage Conditions

Sample Set Spreadsheet

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Samples Preview

Sample ID	!Sample Title	group	Cell type	vaccine	Subject id
GSM733843	2008 LAIV subject ID 1 at D0 post-vaccination	Day0 post vaccination	PBMC	LAIV (FluMist, MedImmune)	1
GSM733844	2008 LAIV subject ID 1 at D3 post-vaccination	Day3 post vaccination	PBMC	LAIV (FluMist, MedImmune)	1
GSM733845	2008 LAIV subject ID 1 at D7 post-vaccination	Day7 post vaccination	PBMC	LAIV (FluMist, MedImmune)	1
GSM733846	2008 LAIV subject ID 5 at D0 post-vaccination	Day0 post vaccination	PBMC	LAIV (FluMist, MedImmune)	5
GSM733847	2008 LAIV subject ID 5 at D3 post-vaccination	Day3 post vaccination	PBMC	LAIV (FluMist, MedImmune)	5

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File
sampleset4000068_sampleannotations_Group.csv Sample Set Spreadsheet
sampleset4000068_sampleannotations_Group.csv Sample Set Spreadsheet

Samples Viewer / Editor

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Sample ID	!Sample Title	Group	Cell type	Vaccine	Subject id	Time point	Hai titer (day 0) - a/south dakota/6/2007 (h1n1)	Hai titer (day 0) - a/uruguay/716/2007 nymc x-175c (h3n2)	Hai titer (day 0) - b/florida 4/2006	Hai titer (day 28) - a/south dakota/6/2007 (h1n1)	Hai titer (day 28) - a/uruguay/716/2007 nymc x-175c (h3n2)	Hai titer (day 28) - b/florida 4/2006
GSM733843	2008 LAIV subject ID 1 at D0 post-vaccination	Day0 post vaccination	PBMC	LAIV (FluMist, MedImmune)	1	D0	40	40	20	40	40	40
GSM733844	2008 LAIV subject ID 1 at D3 post-vaccination	Day3 post vaccination	PBMC	LAIV (FluMist, MedImmune)	1	D3	40	40	20	40	40	40
GSM733845	2008 LAIV subject ID 1 at D7 post-vaccination	Day7 post vaccination	PBMC	LAIV (FluMist, MedImmune)	1	D7	40	40	20	40	40	40
GSM733846	2008 LAIV subject ID 5 at D0 post-vaccination	Day0 post vaccination	PBMC	LAIV (FluMist, MedImmune)	5	D0	5	5	5	5	5	5
GSM733847	2008 LAIV subject ID 5 at D3 post-vaccination	Day3 post vaccination	PBMC	LAIV (FluMist, MedImmune)	5	D3	5	5	5	5	5	5
GSM733848	2008 LAIV subject ID 5 at D7 post-vaccination	Day7 post vaccination	PBMC	LAIV (FluMist, MedImmune)	5	D7	5	5	5	5	5	5
GSM733849	2008 LAIV subject ID 6 at D0 post-vaccination	Day0 post vaccination	PBMC	LAIV (FluMist, MedImmune)	6	D0	5	5	5	5	5	5
GSM733850	2008 LAIV subject ID 6 at D3 post-vaccination	Day3 post vaccination	PBMC	LAIV (FluMist, MedImmune)	6	D3	5	5	5	5	5	5
GSM733851	2008 LAIV subject ID 6 at D7 post-vaccination	Day7 post vaccination	PBMC	LAIV (FluMist, MedImmune)	6	D7	5	5	5	5	5	5
GSM733852	2008 LAIV subject ID 14 at D0 post-vaccination	Day0 post vaccination	PBMC	LAIV (FluMist, MedImmune)	14	D0	5	5	5	5	5	5
GSM733853	2008 LAIV subject ID 14 at D3 post-vaccination	Day3 post vaccination	PBMC	LAIV (FluMist, MedImmune)	14	D3	5	5	5	5	5	5
GSM733854	2008 LAIV subject ID 14 at D7 post-vaccination	Day7 post vaccination	PBMC	LAIV (FluMist, MedImmune)	14	D7	5	5	5	5	5	5
GSM733855	2008 LAIV subject ID 22 at D0 post-vaccination	Day0 post vaccination	PBMC	LAIV (FluMist, MedImmune)	22	D0	5	5	5	5	5	5
GSM733856	2008 LAIV subject ID 22 at D3 post-vaccination	Day3 post vaccination	PBMC	LAIV (FluMist, MedImmune)	22	D3	5	5	5	5	5	5
GSM733857	2008 LAIV subject ID 22 at D7 post-vaccination	Day7 post vaccination	PBMC	LAIV (FluMist, MedImmune)	22	D7	5	5	5	5	5	5
GSM733858	2008 LAIV subject ID 24 at D0 post-vaccination	Day0 post vaccination	PBMC	LAIV (FluMist, MedImmune)	24	D0	5	5	5	5	5	5
GSM733859	2008 LAIV subject ID 24 at D3 post-vaccination	Day3 post vaccination	PBMC	LAIV (FluMist, MedImmune)	24	D3	5	5	5	5	5	5
GSM733860	2008 LAIV subject ID 24 at D7 post-vaccination	Day7 post vaccination	PBMC	LAIV (FluMist, MedImmune)	24	D7	5	5	5	5	5	5
GSM733861	2008 LAIV subject ID 25 at D0 post-vaccination	Day0 post vaccination	PBMC	LAIV (FluMist, MedImmune)	25	D0	320	10	10	320	20	20
GSM733862	2008 LAIV subject ID 25 at D3 post-vaccination	Day3 post vaccination	PBMC	LAIV (FluMist, MedImmune)	25	D3	320	10	10	320	20	20
GSM733863	2008 LAIV subject ID 25 at D7 post-vaccination	Day7 post vaccination	PBMC	LAIV (FluMist, MedImmune)	25	D7	320	10	10	320	20	20
GSM733864	2008 LAIV subject ID 26 at D0 post-vaccination	Day0 post vaccination	PBMC	LAIV (FluMist, MedImmune)	26	D0	20	5	20	5	5	20
GSM733865	2008 LAIV subject ID 26 at D3 post-vaccination	Day3 post vaccination	PBMC	LAIV (FluMist, MedImmune)	26	D3	20	5	20	5	5	20
GSM733866	2008 LAIV subject ID 26 at D7 post-vaccination	Day7 post vaccination	PBMC	LAIV (FluMist, MedImmune)	26	D7	20	5	20	5	5	20
GSM733867	2008 LAIV subject ID 33 at D0 post-vaccination	Day0 post vaccination	PBMC	LAIV (FluMist, MedImmune)	33	D0	5	5	20	5	20	20
GSM733868	2008 LAIV subject ID 33 at D3 post-vaccination	Day3 post vaccination	PBMC	LAIV (FluMist, MedImmune)	33	D3	5	5	20	5	20	20
GSM733869	2008 LAIV subject ID 33 at D7 post-vaccination	Day7 post vaccination	PBMC	LAIV (FluMist, MedImmune)	33	D7	5	5	20	5	20	20
GSM733870	2008 LAIV subject ID 40 at D0 post-vaccination	Day0 post vaccination	PBMC	LAIV (FluMist, MedImmune)	40	D0	80	10	5	80	10	5
GSM733871	2008 LAIV subject ID 40 at D3 post-vaccination	Day3 post vaccination	PBMC	LAIV (FluMist, MedImmune)	40	D3	80	10	5	80	10	5
GSM733872	2008 LAIV subject ID 40 at D7 post-vaccination	Day7 post vaccination	PBMC	LAIV (FluMist, MedImmune)	40	D7	80	10	5	80	10	5
GSM733873	2008 LAIV subject ID 41 at D0 post-vaccination	Day0 post vaccination	PBMC	LAIV (FluMist, MedImmune)	41	D0	5	10	5	5	10	5
GSM733874	2008 LAIV subject ID 41 at D3 post-vaccination	Day3 post vaccination	PBMC	LAIV (FluMist, MedImmune)	41	D3	5	10	5	5	10	5
GSM733875	2008 LAIV subject ID 41 at D7 post-vaccination	Day7 post vaccination	PBMC	LAIV (FluMist, MedImmune)	41	D7	5	10	5	5	10	5
GSM733876	2008 LAIV subject ID 45 at D0 post-vaccination	Day0 post vaccination	PBMC	LAIV (FluMist, MedImmune)	45	D0	5	5	5	5	10	5
GSM733877	2008 LAIV subject ID 45 at D3 post-vaccination	Day3 post vaccination	PBMC	LAIV (FluMist, MedImmune)	45	D3	5	5	5	5	10	5
GSM733878	2008 LAIV subject ID 49 at D0 post-vaccination	Day0 post vaccination	PBMC	LAIV (FluMist, MedImmune)	49	D0	5	5	5	5	5	5
GSM733879	2008 LAIV subject ID 49 at D3 post-vaccination	Day3 post vaccination	PBMC	LAIV (FluMist, MedImmune)	49	D3	5	5	5	5	5	5
GSM733880	2008 LAIV subject ID 49 at D7 post-vaccination	Day7 post vaccination	PBMC	LAIV (FluMist, MedImmune)	49	D7	5	5	5	5	5	5
GSM733881	2008 LAIV subject ID 50 at D0 post-vaccination	Day0 post vaccination	PBMC	LAIV (FluMist, MedImmune)	50	D0	40	20	5	40	20	5
GSM733882	2008 LAIV subject ID 50 at D3 post-vaccination	Day3 post vaccination	PBMC	LAIV (FluMist, MedImmune)	50	D3	40	20	5	40	20	5
GSM733883	2008 LAIV subject ID 50 at D7 post-vaccination	Day7 post vaccination	PBMC	LAIV (FluMist, MedImmune)	50	D7	40	20	5	40	20	5
GSM733884	2008 LAIV subject ID 52 at D0 post-vaccination	Day0 post vaccination	PBMC	LAIV (FluMist, MedImmune)	52	D0	5	20	5	10	20	5
GSM733885	2008 LAIV subject ID 52 at D3 post-vaccination	Day3 post vaccination	PBMC	LAIV (FluMist, MedImmune)	52	D3	5	20	5	10	20	5
GSM733886	2008 LAIV subject ID 52 at D7 post-vaccination	Day7 post vaccination	PBMC	LAIV (FluMist, MedImmune)	52	D7	5	20	5	10	20	5
GSM733887	2008 LAIV subject ID 55 at D0 post-vaccination	Day0 post vaccination	PBMC	LAIV (FluMist, MedImmune)	55	D0	20	160	5	20	160	5
GSM733888	2008 LAIV subject ID 55 at D3 post-vaccination	Day3 post vaccination	PBMC	LAIV (FluMist, MedImmune)	55	D3	20	160	5	20	160	5
GSM733889	2008 LAIV subject ID 55 at D7 post-vaccination	Day7 post vaccination	PBMC	LAIV (FluMist, MedImmune)	55	D7	20	160	5	20	160	5
GSM733890	2008 LAIV subject ID 56 at D0 post-vaccination	Day0 post vaccination	PBMC	LAIV (FluMist, MedImmune)	56	D0	5	5	5	20	5	5
GSM733891	2008 LAIV subject ID 56 at D3 post-vaccination	Day3 post vaccination	PBMC	LAIV (FluMist, MedImmune)	56	D3	5	5	5	20	5	5
GSM733892	2008 LAIV subject ID 56 at D7 post-vaccination	Day7 post vaccination	PBMC	LAIV (FluMist, MedImmune)	56	D7	5	5	5	20	5	5
GSM733893	2008 LAIV subject ID 59 at D0 post-vaccination	Day0 post vaccination	PBMC	LAIV (FluMist, MedImmune)	59	D0	40	20	5	80	20	5
GSM733894	2008 LAIV subject ID 59 at D3 post-vaccination	Day3 post vaccination	PBMC	LAIV (FluMist, MedImmune)	59	D3	40	20	5	80	20	5
GSM733895	2008 LAIV subject ID 59 at D7 post-vaccination	Day7 post vaccination	PBMC	LAIV (FluMist, MedImmune)	59	D7	40	20	5	80	20	5
GSM733896	2008 LAIV subject ID 60 at D0 post-vaccination	Day0 post vaccination	PBMC	LAIV (FluMist, MedImmune)	60	D0	20	5	80	20	5	80
GSM733897	2008 LAIV subject ID 60 at D3 post-vaccination	Day3 post vaccination	PBMC	LAIV (FluMist, MedImmune)	60	D3	20	5	80	20	5	80
GSM733898	2008 LAIV subject ID 60 at D7 post-vaccination	Day7 post vaccination	PBMC	LAIV (FluMist, MedImmune)	60	D7	20	5	80	20	5	80
GSM733899	2008 LAIV subject ID 61 at D0 post-vaccination	Day0 post vaccination	PBMC	LAIV (FluMist, MedImmune)	61	D0	5	40	5	5	40	5
GSM733900	2008 LAIV subject ID 61 at D3 post-vaccination	Day3 post vaccination	PBMC	LAIV (FluMist, MedImmune)	61	D3	5	40	5	5	40	5
GSM733901	2008 LAIV subject ID 61 at D7 post-vaccination	Day7 post vaccination	PBMC	LAIV (FluMist, MedImmune)	61	D7	5	40	5	5	40	5
GSM733902	2008 LAIV subject ID 62 at D0 post-vaccination	Day0 post vaccination	PBMC	LAIV (FluMist, MedImmune)	62	D0	5	5	5	5	5	5
GSM733903	2008 LAIV subject ID 62 at D3 post-vaccination	Day3 post vaccination	PBMC	LAIV (FluMist, MedImmune)	62	D3	5	5	5	5	5	5
GSM733904	2008 LAIV subject ID 62 at D7 post-vaccination	Day7 post vaccination	PBMC	LAIV (FluMist, MedImmune)	62	D7	5	5	5	5	5	5
GSM733905	2008 LAIV subject ID 66 at D0 post-vaccination	Day0 post vaccination	PBMC	LAIV (FluMist, MedImmune)	66	D0	5	5	5	5	5	5
GSM733906	2008 LAIV subject ID 66 at D3 post-vaccination	Day3 post vaccination	PBMC	LAIV (FluMist, MedImmune)	66	D3	5	5	5	5	5	5
GSM733907	2008 LAIV subject ID 66 at D7 post-vaccination	Day7 post vaccination	PBMC	LAIV (FluMist, MedImmune)	66	D7	5	5	5	5	5	5
GSM733908	2008 LAIV subject ID 71 at D0 post-vaccination	Day0 post vaccination	PBMC	LAIV (FluMist, MedImmune)	71	D0	160	5	5	160	5	5
GSM733909	2008 LAIV subject ID 71 at D3 post-vaccination	Day3 post vaccination	PBMC	LAIV (FluMist, MedImmune)	71	D3	160	5	5	160	5	5
GSM733910	2008 LAIV subject ID 71 at D7 post-vaccination	Day7 post vaccination	PBMC	LAIV (FluMist, MedImmune)	71	D7	160	5	5	160	5	5
GSM733911	2008 LAIV subject ID 76 at D0 post-vaccination	Day0 post vaccination	PBMC	LAIV (FluMist, MedImmune)	76	D0	5	5	5	5	80	5
GSM733912	2008 LAIV subject ID 76 at D3 post-vaccination	Day3 post vaccination	PBMC	LAIV (FluMist, MedImmune)	76	D3	5	5	5	5	80	5
GSM733913	2008 LAIV subject ID 76 at D7 post-vaccination	Day7 post vaccination	PBMC	LAIV (FluMist, MedImmune)	76	D7	5	5	5	5	80	5
GSM733914	2008 LAIV subject ID 77 at D0 post-vaccination	Day0 post vaccination	PBMC	LAIV (FluMist, MedImmune)	77	D0	5	5	5	5	5	5
GSM733915	2008 LAIV subject ID 77 at D3 post-vaccination	Day3 post vaccination	PBMC	LAIV (FluMist, MedImmune)	77	D3	5	5	5	5	5	5
GSM733916	2008 LAIV subject ID 77 at D7 post-vaccination	Day7 post vaccination	PBMC	LAIV (FluMist, MedImmune)	77	D7	5	5	5	5	5	5
GSM733917	2008 LAIV subject ID 79 at D0 post-vaccination	Day0 post vaccination	PBMC	LAIV (FluMist, MedImmune)	79	D0	5	5	5	20	5	5
GSM733918	2008 LAIV subject ID 79 at D3 post-vaccination	Day3 post vaccination	PBMC	LAIV (FluMist, MedImmune)	79	D3	5	5	5	20	5	5
GSM733919	2008 LAIV subject ID 79 at D7 post-vaccination	Day7 post vaccination	PBMC	LAIV (FluMist, MedImmune)	79	D7	5	5	5	20	5	5
GSM733920	2008 LAIV subject ID 81 at D0 post-vaccination	Day0 post vaccination	PBMC	LAIV (FluMist, MedImmune)	81	D0	5	5	5	5	5	5
GSM733921	2008 LAIV subject ID 81 at D3 post-vaccination	Day3 post vaccination	PBMC	LAIV (FluMist, MedImmune)	81	D3	5	5	5	5	5	5
GSM733922	2008 LAIV subject ID 81 at D7 post-vaccination	Day7 post vaccination	PBMC	LAIV (FluMist, MedImmune)	81	D7	5	5	5	5	5	5
GSM733923	2008 LAIV subject ID 82 at D0 post-vaccination	Day0 post vaccination	PBMC	LAIV (FluMist, MedImmune)	82	D0	5	5	5	5	5	5
GSM733924	2008 LAIV subject ID 82 at D3 post-vaccination	Day3 post vaccination	PBMC	LAIV (FluMist, MedImmune)	82	D3	5	5	5	5	5	5
GSM733925	2008 LAIV subject ID 82 at D7 post-vaccination	Day7 post vaccination	PBMC	LAIV (FluMist, MedImmune)	82	D7	5	5	5	5	5	5

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