Sample Set Annotation Tool: T cell responses to H1N1v and a longitudinal study of seasonal influenza vaccination

Study Design
Assay
Sample Info
Files
Info
Samples
Group Sets
Analysis

Purpose

Systems biology approach to examine effects of seasonal flu vaccination in adults of different ages on gene expression, cytokine stimulation and serum cytokines with parameters such as immune senescence to uncover new markers and mechanisms behind failure of immune function in many older people.

Hypothesis

Experimental Design

1. Combinatorial tetramer analysis by FACS and/or CyTOF; Phosphoflow. 2. Luminex; Gene expression profiling (Affymetrix microarray). 3. Luminex; Gene expression profiling (Affymetrix microarry); Microneutralization assays. 4. Gene expression profiling (Affymetrix microarray); Immune phenotyping by CyTOF. 5. Gene expression profiling (Affymetrix microarray). 6. Immune phenotyping by CyTOF; Luminex. 7. ELISA.

Experimental Variables

Controls

Methods

Additional Information

Expected Samples:

71 Samples Loaded: 71

Species

Human (Homo sapiens)

Sample Source

Whole Blood

Disease

Influenza vaccine

Treatment Protocol

Growth Protocol

Extraction Protocol

Storage Conditions

Sample Set Spreadsheet

Please log in in order to upload files.

Samples Preview

Sample ID	!Sample Title	tissue	group	Sex	Age (years)
GSM1573121	Ji_Batch5_24Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	female	65.91
GSM1573122	Ji_Batch5_31Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	female	66.86
GSM1573123	Ji_Batch5_60Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	male	30.9
GSM1573124	Ji_Batch5_65Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	female	69.97
GSM1573125	Ji_Batch5_11Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	male	67.71

Please log in in order to upload files.

File
sampleset4000076_sampleannotations_Group.csv Sample Set Spreadsheet
sampleset4000076_sampleannotations_Group.csv Sample Set Spreadsheet

Samples Viewer / Editor

All fields are editable except the "Sample ID" column. To edit a cell, click within the cell. To edit a "date" cell, click on the calendar icon. To cancel an edit, press the ESC key.

Sample ID	!Sample Title	Tissue	Group	Sex	Age (years)	Stanford internal vaccine reference id
GSM1573121	Ji_Batch5_24Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	female	65.91	Fluzone single-dose syringe\|49281-012-50
GSM1573122	Ji_Batch5_31Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	female	66.86	Fluzone single-dose syringe\|49281-012-50
GSM1573123	Ji_Batch5_60Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	male	30.9	Fluzone single-dose syringe\|49281-012-50
GSM1573124	Ji_Batch5_65Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	female	69.97	Fluzone single-dose syringe\|49281-012-50
GSM1573125	Ji_Batch5_11Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	male	67.71	Fluzone single-dose syringe\|49281-012-50
GSM1573126	Ji_Batch5_8Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	male	66.33	Fluzone single-dose syringe\|49281-012-50
GSM1573127	Ji_Batch5_63Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	male	65.63	Fluzone single-dose syringe\|49281-012-50
GSM1573128	Ji_Batch5_4Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	female	33.14	Fluzone single-dose syringe\|49281-012-50
GSM1573129	Ji_Batch5_6Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	female	26.74	Fluzone single-dose syringe\|49281-012-50
GSM1573130	Ji_Batch5_27Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	male	89.05	Fluzone single-dose syringe\|49281-012-50
GSM1573131	Ji_Batch5_21Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	male	88.41	Fluzone single-dose syringe\|49281-012-50
GSM1573132	Ji_Batch5_12Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	female	88.06	Fluzone single-dose syringe\|49281-012-50
GSM1573133	Ji_Batch5_16Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	female	88.63	Fluzone single-dose syringe\|49281-012-50
GSM1573134	Ji_Batch5_67Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	female	89.37	Fluzone single-dose syringe\|49281-012-50
GSM1573135	Ji_Batch5_29Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	male	89.79	Fluzone single-dose syringe\|49281-012-50
GSM1573136	Ji_Batch5_48Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	female	69.48	Fluzone single-dose syringe\|49281-012-50
GSM1573137	Ji_Batch5_47Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	female	82.81	Fluzone single-dose syringe\|49281-012-50
GSM1573138	Ji_Batch5_49Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	male	88.16	Fluzone single-dose syringe\|49281-012-50
GSM1573139	Ji_Batch5_18Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	female	77	Fluzone single-dose syringe\|49281-012-50
GSM1573140	Ji_Batch5_19Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	female	72.13	Fluzone single-dose syringe\|49281-012-50
GSM1573141	Ji_Batch5_25Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	female	89.8	Fluzone single-dose syringe\|49281-012-50
GSM1573142	Ji_Batch5_39Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	male	79.24	Fluzone single-dose syringe\|49281-012-50
GSM1573143	Ji_Batch5_32Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	male	90	Fluzone single-dose syringe\|49281-012-50
GSM1573144	Ji_Batch5_55Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	male	70.55	Fluzone single-dose syringe\|49281-012-50
GSM1573145	Ji_Batch5_70Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	male	76.61	Fluzone single-dose syringe\|49281-012-50
GSM1573146	Ji_Batch5_37Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	female	72.55	Fluzone single-dose syringe\|49281-012-50
GSM1573147	Ji_Batch5_23Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	female	84.72	Fluzone single-dose syringe\|49281-012-50
GSM1573148	Ji_Batch5_34Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	female	90	Fluzone single-dose syringe\|49281-012-50
GSM1573149	Ji_Batch5_66Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	female	87.51	Fluzone single-dose syringe\|49281-012-50
GSM1573150	Ji_Batch5_15Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	female	66.62	Fluzone single-dose syringe\|49281-012-50
GSM1573151	Ji_Batch5_26Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	female	81.25	Fluzone single-dose syringe\|49281-012-50
GSM1573152	Ji_Batch5_42Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	female	82.59	Fluzone single-dose syringe\|49281-012-50
GSM1573153	Ji_Batch5_41Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	female	82.68	Fluzone single-dose syringe\|49281-012-50
GSM1573154	Ji_Batch5_9Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	female	70.67	Fluzone single-dose syringe\|49281-012-50
GSM1573155	Ji_Batch5_59Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	male	87.93	Fluzone single-dose syringe\|49281-012-50
GSM1573156	Ji_Batch5_28Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	male	77.24	Fluzone single-dose syringe\|49281-012-50
GSM1573157	Ji_Batch5_57Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	female	73.03	Fluzone single-dose syringe\|49281-012-50
GSM1573158	Ji_Batch5_40Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	female	30.4	Fluzone single-dose syringe\|49281-012-50
GSM1573159	Ji_Batch5_35Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	male	79.07	Fluzone single-dose syringe\|49281-012-50
GSM1573160	Ji_Batch5_36Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	male	90	Fluzone single-dose syringe\|49281-012-50
GSM1573161	Ji_Batch5_3Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	male	75.55	Fluzone single-dose syringe\|49281-012-50
GSM1573162	Ji_Batch5_5Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	male	81.57	Fluzone single-dose syringe\|49281-012-50
GSM1573163	Ji_Batch5_2Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	female	65.71	Fluzone single-dose syringe\|49281-012-50
GSM1573164	Ji_Batch5_22Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	female	90	Fluzone single-dose syringe\|49281-012-50
GSM1573165	Ji_Batch5_43Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	male	29.48	Fluzone single-dose syringe\|49281-012-50
GSM1573166	Ji_Batch5_69Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	female	90	Fluzone single-dose syringe\|49281-012-50
GSM1573167	Ji_Batch5_44Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	female	65.13	Fluzone single-dose syringe\|49281-012-50
GSM1573168	Ji_Batch5_64Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	male	90	Fluzone single-dose syringe\|49281-012-50
GSM1573169	Ji_Batch5_30Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	female	88.32	Fluzone single-dose syringe\|49281-012-50
GSM1573170	Ji_Batch5_61Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	female	86.11	Fluzone single-dose syringe\|49281-012-50
GSM1573171	Ji_Batch5_1Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	female	33.16	Fluzone single-dose syringe\|49281-012-50
GSM1573172	Ji_Batch5_10Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	male	31.26	Fluzone single-dose syringe\|49281-012-50
GSM1573173	Ji_Batch5_46Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	male	27.18	Fluzone single-dose syringe\|49281-012-50
GSM1573174	Ji_Batch5_68Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	female	32	Fluzone single-dose syringe\|49281-012-50
GSM1573175	Ji_Batch5_53Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	female	88.18	Fluzone single-dose syringe\|49281-012-50
GSM1573176	Ji_Batch5_20Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	female	75.28	Fluzone single-dose syringe\|49281-012-50
GSM1573177	Ji_Batch5_38Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	female	86.79	Fluzone single-dose syringe\|49281-012-50
GSM1573178	Ji_Batch5_7Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	male	26.36	Fluzone single-dose syringe\|49281-012-50
GSM1573179	Ji_Batch5_58Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	male	80.17	Fluzone single-dose syringe\|49281-012-50
GSM1573180	Ji_Batch5_56Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	female	86.63	Fluzone single-dose syringe\|49281-012-50
GSM1573181	Ji_Batch5_62Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	male	84.52	Fluzone single-dose syringe\|49281-012-50
GSM1573182	Ji_Batch5_71Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	female	27.52	Fluzone single-dose syringe\|49281-012-50
GSM1573183	Ji_Batch5_54Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	male	30.08	Fluzone single-dose syringe\|49281-012-50
GSM1573184	Ji_Batch5_45Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	female	21.39	Fluzone single-dose syringe\|49281-012-50
GSM1573185	Ji_Batch5_14Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	female	27.98	Fluzone single-dose syringe\|49281-012-50
GSM1573186	Ji_Batch5_17Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	male	26.74	Fluzone single-dose syringe\|49281-012-50
GSM1573187	Ji_Batch5_33Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	male	25.17	Fluzone single-dose syringe\|49281-012-50
GSM1573188	Ji_Batch5_50Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	male	22.51	Fluzone single-dose syringe\|49281-012-50
GSM1573189	Ji_Batch5_51Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	female	77.12	Fluzone single-dose syringe\|49281-012-50
GSM1573190	Ji_Batch5_13Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	female	31.61	Fluzone single-dose syringe\|49281-012-50
GSM1573191	Ji_Batch5_52Baseline (day 0)	whole blood	Day0 Fluzone single dose syringe	female	25.41	Fluzone single-dose syringe\|49281-012-50

Group Sets View in Gene Expression Browser

Name
All Samples	Default	View
In order to make new Group Sets or change settings, please log in.

Module Analysis

Group Set
All Samples

SIDRA