Sample Set Annotation Tool: T cell responses to H1N1v and a longitudinal study of seasonal influenza vaccination

Study Design
Assay
Sample Info
Files
Info
Samples
Group Sets
Analysis

Purpose

Systems biology approach to examine effects of seasonal flu vaccination in adults of different ages on gene expression, cytokine stimulation and serum cytokines with parameters such as immune senescence to uncover new markers and mechanisms behind failure of immune function in many older people.

Hypothesis

Experimental Design

Total RNA was extracted from whole-blood lysates obtained from individuals immediately prior to vaccination against seasonal influenza, followed by depletion of globin messenger RNA. The goal was to evaluate potential gene expression signatures at Day 0 associated with immune senescence.

Experimental Variables

Controls

Methods

Additional Information

Expected Samples:

72 Samples Loaded: 72

Species

Human (Homo sapiens)

Sample Source

Whole Blood

Disease

Influenza vaccine

Treatment Protocol

Growth Protocol

Extraction Protocol

Storage Conditions

Sample Set Spreadsheet

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Samples Preview

Sample ID	!Sample Title	group	time	race	vaccine
GSM1422706	Baseline (day 0) 5863981123_C	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe
GSM1422707	Baseline (day 0) 5863981123_F	Fluzone single dose syringe Baseline	Baseline- D0	Other	Fluzone single-dose syringe
GSM1422708	Baseline (day 0) 5863981123_D	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe
GSM1422709	Baseline (day 0) 5863981123_E	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe
GSM1422710	Baseline (day 0) 5863981123_G	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe

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File
sampleset4000033_sampleannotations_Group.csv Sample Set Spreadsheet
sampleset4000033_sampleannotations_Group.csv Sample Set Spreadsheet

Samples Viewer / Editor

All fields are editable except the "Sample ID" column. To edit a cell, click within the cell. To edit a "date" cell, click on the calendar icon. To cancel an edit, press the ESC key.

Sample ID	!Sample Title	Group	Time	Race	Vaccine	Sex
GSM1422706	Baseline (day 0) 5863981123_C	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	male
GSM1422707	Baseline (day 0) 5863981123_F	Fluzone single dose syringe Baseline	Baseline- D0	Other	Fluzone single-dose syringe	female
GSM1422708	Baseline (day 0) 5863981123_D	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	female
GSM1422709	Baseline (day 0) 5863981123_E	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	male
GSM1422710	Baseline (day 0) 5863981123_G	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	female
GSM1422711	Baseline (day 0) 5863981123_J	Fluzone single dose syringe Baseline	Baseline- D0	Asian	Fluzone single-dose syringe	female
GSM1422712	Baseline (day 0) 5863981123_K	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	female
GSM1422713	Baseline (day 0) 5863981123_B	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	female
GSM1422714	Baseline (day 0) 5863981123_A	Fluzone single dose syringe Baseline	Baseline- D0	Other	Fluzone single-dose syringe	female
GSM1422715	Baseline (day 0) 5863981123_I	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	female
GSM1422716	Baseline (day 0) 5863981123_L	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	male
GSM1422717	Baseline (day 0) 5863981124_H	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	female
GSM1422718	Baseline (day 0) 5863981124_B	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	female
GSM1422719	Baseline (day 0) 5863981124_I	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	male
GSM1422720	Baseline (day 0) 5863981124_L	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	female
GSM1422721	Baseline (day 0) 5863981124_G	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	male
GSM1422722	Baseline (day 0) 5863981124_J	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	female
GSM1422723	Baseline (day 0) 5863981124_E	Fluzone single dose syringe Baseline	Baseline- D0	Other	Fluzone single-dose syringe	female
GSM1422724	Baseline (day 0) 5863981124_A	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	female
GSM1422725	Baseline (day 0) 5863981124_K	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	male
GSM1422726	Baseline (day 0) 5863981124_F	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	male
GSM1422727	Baseline (day 0) 5863981124_D	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	female
GSM1422728	Baseline (day 0) 5863981130_B	Fluzone single dose syringe Baseline	Baseline- D0	Asian	Fluzone single-dose syringe	male
GSM1422729	Baseline (day 0) 5863981130_D	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	male
GSM1422730	Baseline (day 0) 5863981130_H	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	female
GSM1422731	Baseline (day 0) 5863981130_A	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	male
GSM1422732	Baseline (day 0) 5863981130_C	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	female
GSM1422733	Baseline (day 0) 5863981130_F	Fluzone single dose syringe Baseline	Baseline- D0	Other	Fluzone single-dose syringe	female
GSM1422734	Baseline (day 0) 5863981130_L	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	female
GSM1422735	Baseline (day 0) 5863981130_K	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	female
GSM1422736	Baseline (day 0) 5863981130_I	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	male
GSM1422737	Baseline (day 0) 5863981130_E	Fluzone single dose syringe Baseline	Baseline- D0	Asian	Fluzone single-dose syringe	male
GSM1422738	Baseline (day 0) 5863981130_J	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	male
GSM1422739	Baseline (day 0) 5863981132_B	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	female
GSM1422740	Baseline (day 0) 5863981132_L	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	male
GSM1422741	Baseline (day 0) 5863981132_H	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	female
GSM1422742	Baseline (day 0) 5863981132_D	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	female
GSM1422743	Baseline (day 0) 5863981132_C	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	female
GSM1422744	Baseline (day 0) 5863981132_A	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	male
GSM1422745	Baseline (day 0) 5863981132_G	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	female
GSM1422746	Baseline (day 0) 5863981132_F	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	female
GSM1422747	Baseline (day 0) 5863981132_J	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	female
GSM1422748	Baseline (day 0) 5863981133_E	Fluzone single dose syringe Baseline	Baseline- D0	Asian	Fluzone single-dose syringe	female
GSM1422749	Baseline (day 0) 5863981133_I	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	male
GSM1422750	Baseline (day 0) 5863981133_F	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	female
GSM1422751	Baseline (day 0) 5863981133_G	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	female
GSM1422752	Baseline (day 0) 5863981133_B	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	male
GSM1422753	Baseline (day 0) 5863981133_H	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	female
GSM1422754	Baseline (day 0) 5863981133_K	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	male
GSM1422755	Baseline (day 0) 5863981133_A	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	female
GSM1422756	Baseline (day 0) 5863981133_C	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	male
GSM1422757	Baseline (day 0) 5863981133_D	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	female
GSM1422758	Baseline (day 0) 5863981133_L	Fluzone single dose syringe Baseline	Baseline- D0	Asian	Fluzone single-dose syringe	male
GSM1422759	Baseline (day 0) 5863981135_H	Fluzone single dose syringe Baseline	Baseline- D0	Other	Fluzone single-dose syringe	male
GSM1422760	Baseline (day 0) 5863981135_E	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	male
GSM1422761	Baseline (day 0) 5863981135_I	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	female
GSM1422762	Baseline (day 0) 5863981135_A	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	male
GSM1422763	Baseline (day 0) 5863981135_G	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	male
GSM1422764	Baseline (day 0) 5863981135_K	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	female
GSM1422765	Baseline (day 0) 5863981135_L	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	female
GSM1422766	Baseline (day 0) 5863981135_F	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	male
GSM1422767	Baseline (day 0) 5863981135_C	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	female
GSM1422768	Baseline (day 0) 5863981135_J	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	female
GSM1422769	Baseline (day 0) 5863981135_D	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	female
GSM1422770	Baseline (day 0) 5863981135_B	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	male
GSM1422771	Baseline (day 0) 5863981136_F	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	female
GSM1422772	Baseline (day 0) 5863981136_C	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	female
GSM1422773	Baseline (day 0) 5863981136_E	Fluzone single dose syringe Baseline	Baseline- D0	Other	Fluzone single-dose syringe	male
GSM1422774	Baseline (day 0) 5863981136_B	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	female
GSM1422775	Baseline (day 0) 5863981136_A	Fluzone single dose syringe Baseline	Baseline- D0	White	Fluzone single-dose syringe	female
GSM1422776	Baseline (day 0) 5863981136_G	Fluzone single dose syringe Baseline	Baseline- D0	Asian	Fluzone single-dose syringe	female
GSM1422777	Baseline (day 0) 5863981136_D	Fluzone single dose syringe Baseline	Baseline- D0	Asian	Fluzone single-dose syringe	male

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Module Analysis

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