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Genome-wide profiling of whole blood from healthy adult volunteers before and after receiving non-live vaccines including seasonal influenza or pneumococcal vaccine or placebo (saline) injections II. GSE48762_Finger prick-test set.

In Progress
Clinical

GSE48762

Purpose

The objective of this study is to: 1) Characterize the immune responsiveness to administration of non-live vaccines in three cohorts of healthy adult subjects through the analysis of blood leukocytes transcriptional profiles. 2) Validate whole blood transcriptional profiles generated from standard 3mL blood draws versus 200uL blood draws obtained by finger stick. 3) Discover potential biomarkers for immune-responsiveness to non-live vaccines.

Hypothesis

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Experimental Design

A total of 621 blood samples were collected either by venipuncture (387) or finger prick (234) from four groups of healthy adults receiving either, 2009/10 seasonal influenza or 23-valent pneumococcal vaccine or placebo (saline) injections. Subjects were recruited in 3 cohorts with 4-7 individuals per group; cohort 3 was recruited for validation of the systemic day 1 immune signature in response to seasonal influenza and pneumococcal vaccination. From each subject, peripheral blood was drawn into Tempus tubes (Applied Biosystems) or obtained by finger prick into micro capillaries and then transferred into tempus reagent to lyse blood cells and stabilize RNA before storing at -80ºC until mRNA extraction. The training set for transcriptional profiling of blood obtained by venipuncture was performed in cohort 1 which included 6 healthy adult individuals receiving seasonal influenza vaccine, 6 healthy adult individuals receiving pneumococcal vaccine, and 6 healthy adult individuals receiving placebo (saline injections). The test set for transcriptional profiling of blood obtained by venipuncture was performed in cohort 2 which included 6 healthy adult individuals receiving seasonal influenza vaccine, 6 healthy adult individuals receiving pneumococcal vaccine, and 6 healthy adult individuals receiving placebo (saline injections). The validation set for confirming systemic day 1 transcriptome immune signature in response to seasonal influenza and pneumococcal vaccination was performed in cohort 3 which included 6 healthy adult individuals receiving seasonal influenza vaccine and 4 healthy adult individuals receiving pneumococcal vaccine. The training set for transcriptional profiling of blood obtained by finger prick was performed in cohort 2 and the test set in cohort 1.

Experimental Variables

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Controls

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Methods

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Additional Information

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Microarray
Illumina HumanHT-12 v3
49 Samples Loaded: 49
Human (Homo sapiens)
Whole Blood
Influenza
Sample Set Spreadsheet
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Samples Preview
Sample ID !Sample Title Age Ethnicity Gender group
GSM745399 009-282-018-TP2 [5447320035B] 23 Non Hispanic or Latino F FLUZONE day0
GSM745400 009-282-010-TP1 [5447320035G] 29 Non Hispanic or Latino M FLUZONE day-7
GSM745401 009-282-024-TP1 [5447320036A] 39 M FLUZONE day-7
GSM745402 009-282-010-TP13 [5447320036B] 29 Non Hispanic or Latino M FLUZONE day7
GSM745403 009-282-018-TP1 [5447320036C] 23 Non Hispanic or Latino F FLUZONE day-7

Upload Files

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Browse Files

File
sampleset4000038_sampleannotations_Group.csv
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Clinical Datasource
Links
http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE48762
http://www.ncbi.nlm.nih.gov/pubmed?LinkName=gds_pubmed&from_uid=200048762

Samples Viewer / Editor

All fields are editable except the "Sample ID" column. To edit a cell, click within the cell. To edit a "date" cell, click on the calendar icon. To cancel an edit, press the ESC key.

Sample ID !Sample Title Age Ethnicity Gender Group Day Race Vaccine Subject id Blood source Sample set
GSM745399
009-282-018-TP2 [5447320035B]
23
Non Hispanic or Latino
F
FLUZONE day0
0
Caucasian
FLUZONE
18
Finger
Test_Set_Finger
GSM745400
009-282-010-TP1 [5447320035G]
29
Non Hispanic or Latino
M
FLUZONE day-7
-7
Caucasian
FLUZONE
10
Finger
Test_Set_Finger
GSM745401
009-282-024-TP1 [5447320036A]
39
 
M
FLUZONE day-7
-7
Asian
FLUZONE
24
Finger
Test_Set_Finger
GSM745402
009-282-010-TP13 [5447320036B]
29
Non Hispanic or Latino
M
FLUZONE day7
7
Caucasian
FLUZONE
10
Finger
Test_Set_Finger
GSM745403
009-282-018-TP1 [5447320036C]
23
Non Hispanic or Latino
F
FLUZONE day-7
-7
Caucasian
FLUZONE
18
Finger
Test_Set_Finger
GSM745404
009-282-010-TP2 [5447320036J]
29
Non Hispanic or Latino
M
FLUZONE day0
0
Caucasian
FLUZONE
10
Finger
Test_Set_Finger
GSM745405
009-282-001-TP13 [5447320037B]
49
 
F
FLUZONE day7
7
Asian
FLUZONE
1
Finger
Test_Set_Finger
GSM745406
009-282-022-TP2 [5447320037F]
32
 
F
FLUZONE day0
0
Asian
FLUZONE
22
Finger
Test_Set_Finger
GSM745407
009-282-022-TP1 [5447320038C]
32
 
F
FLUZONE day-7
-7
Asian
FLUZONE
22
Finger
Test_Set_Finger
GSM745408
009-282-001-TP2 [5447320038E]
49
 
F
FLUZONE day0
0
Asian
FLUZONE
1
Finger
Test_Set_Finger
GSM745409
009-282-024-TP13 [5447320038F]
39
 
M
FLUZONE day7
7
Asian
FLUZONE
24
Finger
Test_Set_Finger
GSM745410
009-282-022-TP13 [5447320038G]
32
 
F
FLUZONE day7
7
Asian
FLUZONE
22
Finger
Test_Set_Finger
GSM745411
009-282-001-TP1 [5447320039D]
49
 
F
FLUZONE day-7
-7
Asian
FLUZONE
1
Finger
Test_Set_Finger
GSM745412
009-282-008-TP1 [5447320039F]
29
Non Hispanic or Latino
M
FLUZONE day-7
-7
Caucasian
FLUZONE
8
Finger
Test_Set_Finger
GSM745413
009-282-024-TP2 [5447320039I]
39
 
M
FLUZONE day0
0
Asian
FLUZONE
24
Finger
Test_Set_Finger
GSM745414
009-282-018-TP13 [5447320039K]
23
Non Hispanic or Latino
F
FLUZONE day7
7
Caucasian
FLUZONE
18
Finger
Test_Set_Finger
GSM745415
009-282-008-TP13 [5447320039L]
29
Non Hispanic or Latino
M
FLUZONE day7
7
Caucasian
FLUZONE
8
Finger
Test_Set_Finger
GSM745416
009-282-006-TP13 [5447320035E]
24
 
F
NS day7
7
African American
NS
6
Finger
Test_Set_Finger
GSM745417
009-282-006-TP1 [5447320035J]
24
 
F
NS day-7
-7
African American
NS
6
Finger
Test_Set_Finger
GSM745418
009-282-013-TP2 [5447320035K]
30
Non Hispanic or Latino
F
NS day0
0
Caucasian
NS
13
Finger
Test_Set_Finger
GSM745419
009-282-003-TP2 [5447320035L]
31
Hispanic
F
NS day0
0
Caucasian
NS
3
Finger
Test_Set_Finger
GSM745420
009-282-006-TP2 [5447320036I]
24
 
F
NS day0
0
African American
NS
6
Finger
Test_Set_Finger
GSM745421
009-282-014-TP13 [5447320036K]
26
Non Hispanic or Latino
M
NS day7
7
Caucasian
NS
14
Finger
Test_Set_Finger
GSM745422
009-282-013-TP1 [5447320036L]
30
Non Hispanic or Latino
F
NS day-7
-7
Caucasian
NS
13
Finger
Test_Set_Finger
GSM745423
009-282-014-TP1 [5447320037C]
26
Non Hispanic or Latino
M
NS day-7
-7
Caucasian
NS
14
Finger
Test_Set_Finger
GSM745424
009-282-020-TP2 [5447320037D]
28
Non Hispanic or Latino
M
NS day0
0
Caucasian
NS
20
Finger
Test_Set_Finger
GSM745425
009-282-003-TP13 [5447320038B]
31
Hispanic
F
NS day7
7
Caucasian
NS
3
Finger
Test_Set_Finger
GSM745426
009-282-003-TP1 [5447320038K]
31
Hispanic
F
NS day-7
-7
Caucasian
NS
3
Finger
Test_Set_Finger
GSM745427
009-282-013-TP13 [5447320039C]
30
Non Hispanic or Latino
F
NS day7
7
Caucasian
NS
13
Finger
Test_Set_Finger
GSM745428
009-282-020-TP13 [5447320040B]
28
Non Hispanic or Latino
M
NS day7
7
Caucasian
NS
20
Finger
Test_Set_Finger
GSM745429
009-282-014-TP2 [5447320040G]
26
Non Hispanic or Latino
M
NS day0
0
Caucasian
NS
14
Finger
Test_Set_Finger
GSM745430
009-282-007-TP2 [5447320035D]
25
Non Hispanic or Latino
F
PNEUM day0
0
Caucasian
PNEUM
7
Finger
Test_Set_Finger
GSM745431
009-282-004-TP13 [5447320035F]
22
Non Hispanic or Latino
F
PNEUM day7
7
Caucasian
PNEUM
4
Finger
Test_Set_Finger
GSM745432
009-282-007-TP1 [5447320035H]
25
Non Hispanic or Latino
F
PNEUM day-7
-7
Caucasian
PNEUM
7
Finger
Test_Set_Finger
GSM745433
009-282-015-TP13 [5447320036F]
25
Hispanic
M
PNEUM day7
7
Caucasian
PNEUM
15
Finger
Test_Set_Finger
GSM745434
009-282-015-TP1 [5447320037E]
25
Hispanic
M
PNEUM day-7
-7
Caucasian
PNEUM
15
Finger
Test_Set_Finger
GSM745435
009-282-021-TP1 [5447320037J]
30
Non Hispanic or Latino
M
PNEUM day-7
-7
Caucasian
PNEUM
21
Finger
Test_Set_Finger
GSM745436
009-282-026-TP1 [5447320037K]
29
Non Hispanic or Latino
F
PNEUM day-7
-7
Caucasian
PNEUM
26
Finger
Test_Set_Finger
GSM745437
009-282-021-TP2 [5447320037L]
30
Non Hispanic or Latino
M
PNEUM day0
0
Caucasian
PNEUM
21
Finger
Test_Set_Finger
GSM745438
009-282-011-TP2 [5447320038A]
27
 
M
PNEUM day0
0
African American
PNEUM
11
Finger
Test_Set_Finger
GSM745439
009-282-026-TP13 [5447320038I]
29
Non Hispanic or Latino
F
PNEUM day7
7
Caucasian
PNEUM
26
Finger
Test_Set_Finger
GSM745440
009-282-026-TP2 [5447320038L]
29
Non Hispanic or Latino
F
PNEUM day0
0
Caucasian
PNEUM
26
Finger
Test_Set_Finger
GSM745441
009-282-007-TP13 [5447320039E]
25
Non Hispanic or Latino
F
PNEUM day7
7
Caucasian
PNEUM
7
Finger
Test_Set_Finger
GSM745442
009-282-021-TP13 [5447320040A]
30
Non Hispanic or Latino
M
PNEUM day7
7
Caucasian
PNEUM
21
Finger
Test_Set_Finger
GSM745443
009-282-015-TP2 [5447320040D]
25
Hispanic
M
PNEUM day0
0
Caucasian
PNEUM
15
Finger
Test_Set_Finger
GSM745444
009-282-011-TP13 [5447320040E]
27
 
M
PNEUM day7
7
African American
PNEUM
11
Finger
Test_Set_Finger
GSM745445
009-282-004-TP1 [5447320040F]
22
Non Hispanic or Latino
F
PNEUM day-7
-7
Caucasian
PNEUM
4
Finger
Test_Set_Finger
GSM745446
009-282-011-TP1 [5447320040H]
27
 
M
PNEUM day-7
-7
African American
PNEUM
11
Finger
Test_Set_Finger
GSM745447
009-282-004-TP2 [5447320040I]
22
Non Hispanic or Latino
F
PNEUM day0
0
Caucasian
PNEUM
4
Finger
Test_Set_Finger

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