Sample Set Annotation Tool: Genome-wide profiling of whole blood from healthy adult volunteers before and after receiving non-live vaccines including seasonal influenza or pneumococcal vaccine or placebo (saline) injections I. GSE30059

Study Design
Assay
Sample Info
Files
Info
Samples
Group Sets
Analysis

Purpose

The objective of this study is to: 1) Characterize the cellular origin of transciptional signatures observed on day 1 after vaccination with 2009/10 seasonal influenza and pneumococcal vaccine discovered by transcriptional profiling of whole blood samples in data set “WholeBlood_SysVax”. 2) Discover potential biomarkers for immune-responsiveness to non-live vaccines.

Hypothesis

Experimental Design

In this Series, a total of 72 samples of leukocyte subsets (neutrophils, monocytes, CD4+ T and CD8+ T lymphocytes) were isolated before and 24 hours after vaccination from 6 healthy adult individuals receiving seasonal influenza and 4 healthy adult individuals receiving pneumococcal vaccine. From each subject, neutrophils and peripheral blood mononuclear cells were obtained by Ficoll gradient separation and then CD14+ monocytes, CD4+ T and CD8+ T cells were purified by sequential positive bead selection. Cells were transferred into RLT buffer and stored at -80ºC until mRNA extraction.

Experimental Variables

Controls

Methods

Additional Information

Expected Samples:

72 Samples Loaded: 72

Species

Human (Homo sapiens)

Sample Source

Whole Blood

Disease

Influenza

Treatment Protocol

Growth Protocol

Extraction Protocol

Storage Conditions

Sample Set Spreadsheet

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Samples Preview

Sample ID	!Sample Title	Age	Ethnicity	Gender	group
GSM744141	009_179_045_CD14_D0	45	Not Hispanic or Latino	M	Pneumovax day0 CD14p
GSM744142	009_179_041_CD14_D1	40	Not Hispanic or Latino	M	Flu vaccine day1 CD14p
GSM744143	009_179_044_CD14_D1	23	Not Hispanic or Latino	F	Flu vaccine day1 CD14p
GSM744144	009_179_040_CD14_D0	44		F	Flu vaccine day0 CD14p
GSM744145	009_179_045_CD14_D1	45	Not Hispanic or Latino	M	Pneumovax day1 CD14p

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File
sampleset4000037_sampleannotations_Group.csv Sample Set Spreadsheet
sampleset4000037_sampleannotations_Group.csv Sample Set Spreadsheet
sampleset4000037_sampleannotations_Group.csv Sample Set Spreadsheet
sampleset4000037_sampleannotations_Group.csv Sample Set Spreadsheet

Samples Viewer / Editor

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Sample ID	!Sample Title	Age	Ethnicity	Gender	Group	Cell Population	Day	Race	Vaccine	Subject id
GSM744141	009_179_045_CD14_D0	45	Not Hispanic or Latino	M	Pneumovax day0 CD14p	CD14p	D0	Caucasian	Pneumovax vaccine group	45
GSM744142	009_179_041_CD14_D1	40	Not Hispanic or Latino	M	Flu vaccine day1 CD14p	CD14p	D1	Caucasian	Influenza vaccine group	41
GSM744143	009_179_044_CD14_D1	23	Not Hispanic or Latino	F	Flu vaccine day1 CD14p	CD14p	D1	Caucasian	Influenza vaccine group	44
GSM744144	009_179_040_CD14_D0	44		F	Flu vaccine day0 CD14p	CD14p	D0	African American	Influenza vaccine group	40
GSM744145	009_179_045_CD14_D1	45	Not Hispanic or Latino	M	Pneumovax day1 CD14p	CD14p	D1	Caucasian	Pneumovax vaccine group	45
GSM744146	009_179_043_CD14_D1	36	Not Hispanic or Latino	F	Flu vaccine day1 CD14p	CD14p	D1	Caucasian	Influenza vaccine group	43
GSM744147	009_179_047_CD14_D1	23	Not Hispanic or Latino	M	Pneumovax day1 CD14p	CD14p	D1	Caucasian	Pneumovax vaccine group	47
GSM744148	009_179_047_CD14_D0	23	Not Hispanic or Latino	M	Pneumovax day0 CD14p	CD14p	D0	Caucasian	Pneumovax vaccine group	47
GSM744149	009_179_048_CD14_D1	40	Hispanic	F	Pneumovax day1 CD14p	CD14p	D1	Caucasian	Pneumovax vaccine group	48
GSM744150	009_179_042_CD14_D0	26	Not Hispanic or Latino	M	Flu vaccine day0 CD14p	CD14p	D0	Caucasian	Influenza vaccine group	42
GSM744151	009_179_046_CD14_D0	31	Not Hispanic or Latino	F	Pneumovax day0 CD14p	CD14p	D0	Caucasian	Pneumovax vaccine group	46
GSM744152	009_179_042_CD14_D1	26	Not Hispanic or Latino	M	Flu vaccine day1 CD14p	CD14p	D1	Caucasian	Influenza vaccine group	42
GSM744153	009_179_041_CD14_D0	40	Not Hispanic or Latino	M	Flu vaccine day0 CD14p	CD14p	D0	Caucasian	Influenza vaccine group	41
GSM744154	009_179_044_CD14_D0	23	Not Hispanic or Latino	F	Flu vaccine day0 CD14p	CD14p	D0	Caucasian	Influenza vaccine group	44
GSM744155	009_179_039_CD14_D1	26	Not Hispanic or Latino	M	Flu vaccine day1 CD14p	CD14p	D1	Caucasian	Influenza vaccine group	39
GSM744156	009_179_039_CD14_D0	26	Not Hispanic or Latino	M	Flu vaccine day0 CD14p	CD14p	D0	Caucasian	Influenza vaccine group	39
GSM744157	009_179_048_CD14_D0	40	Hispanic	F	Pneumovax day0 CD14p	CD14p	D0	Caucasian	Pneumovax vaccine group	48
GSM744158	009_179_046_CD14_D1	31	Not Hispanic or Latino	F	Pneumovax day1 CD14p	CD14p	D1	Caucasian	Pneumovax vaccine group	46
GSM744159	009_179_040_CD14_D1	44		F	Flu vaccine day1 CD14p	CD14p	D1	African American	Influenza vaccine group	40
GSM744160	009_179_043_CD14_D0	36	Not Hispanic or Latino	F	Flu vaccine day0 CD14p	CD14p	D0	Caucasian	Influenza vaccine group	43
GSM744161	009_179_041_CD4_D0	40	Not Hispanic or Latino	M	Flu vaccine day0 CD4p	CD4p	D0	Caucasian	Influenza vaccine group	41
GSM744162	009_179_041_CD4_D1	40	Not Hispanic or Latino	M	Flu vaccine day1 CD4p	CD4p	D1	Caucasian	Influenza vaccine group	41
GSM744163	009_179_047_CD4_D1	23	Not Hispanic or Latino	M	Pneumovax day1 CD4p	CD4p	D1	Caucasian	Pneumovax vaccine group	47
GSM744164	009_179_044_CD4_D1	23	Not Hispanic or Latino	F	Flu vaccine day1 CD4p	CD4p	D1	Caucasian	Influenza vaccine group	44
GSM744165	009_179_047_CD4_D0	23	Not Hispanic or Latino	M	Pneumovax day0 CD4p	CD4p	D0	Caucasian	Pneumovax vaccine group	47
GSM744166	009_179_042_CD4_D0	26	Not Hispanic or Latino	M	Flu vaccine day0 CD4p	CD4p	D0	Caucasian	Influenza vaccine group	42
GSM744167	009_179_042_CD4_D1	26	Not Hispanic or Latino	M	Flu vaccine day1 CD4p	CD4p	D1	Caucasian	Influenza vaccine group	42
GSM744168	009_179_043_CD4_D0	36	Not Hispanic or Latino	F	Flu vaccine day0 CD4p	CD4p	D0	Caucasian	Influenza vaccine group	43
GSM744169	009_179_044_CD4_D0	23	Not Hispanic or Latino	F	Flu vaccine day0 CD4p	CD4p	D0	Caucasian	Influenza vaccine group	44
GSM744170	009_179_040_CD4_D0	44		F	Flu vaccine day0 CD4p	CD4p	D0	African American	Influenza vaccine group	40
GSM744171	009_179_043_CD4_D1	36	Not Hispanic or Latino	F	Flu vaccine day1 CD4p	CD4p	D1	Caucasian	Influenza vaccine group	43
GSM744172	009_179_040_CD4_D1	44		F	Flu vaccine day1 CD4p	CD4p	D1	African American	Influenza vaccine group	40
GSM744173	009_179_039_CD8_D1	26	Not Hispanic or Latino	M	Flu vaccine day1 CD8p	CD8p	D1	Caucasian	Influenza vaccine group	39
GSM744174	009_179_041_CD8_D1	40	Not Hispanic or Latino	M	Flu vaccine day1 CD8p	CD8p	D1	Caucasian	Influenza vaccine group	41
GSM744175	009_179_039_CD8_D0	26	Not Hispanic or Latino	M	Flu vaccine day0 CD8p	CD8p	D0	Caucasian	Influenza vaccine group	39
GSM744176	009_179_048_CD8_D1	40	Hispanic	F	Pneumovax day1 CD8p	CD8p	D1	Caucasian	Pneumovax vaccine group	48
GSM744177	009_179_043_CD8_D1	36	Not Hispanic or Latino	F	Flu vaccine day1 CD8p	CD8p	D1	Caucasian	Influenza vaccine group	43
GSM744178	009_179_044_CD8_D0	23	Not Hispanic or Latino	F	Flu vaccine day0 CD8p	CD8p	D0	Caucasian	Influenza vaccine group	44
GSM744179	009_179_045_CD8_D1	45	Not Hispanic or Latino	M	Pneumovax day1 CD8p	CD8p	D1	Caucasian	Pneumovax vaccine group	45
GSM744180	009_179_047_CD8_D1	23	Not Hispanic or Latino	M	Pneumovax day1 CD8p	CD8p	D1	Caucasian	Pneumovax vaccine group	47
GSM744181	009_179_044_CD8_D1	23	Not Hispanic or Latino	F	Flu vaccine day1 CD8p	CD8p	D1	Caucasian	Influenza vaccine group	44
GSM744182	009_179_047_CD8_D0	23	Not Hispanic or Latino	M	Pneumovax day0 CD8p	CD8p	D0	Caucasian	Pneumovax vaccine group	47
GSM744183	009_179_042_CD8_D1	26	Not Hispanic or Latino	M	Flu vaccine day1 CD8p	CD8p	D1	Caucasian	Influenza vaccine group	42
GSM744184	009_179_046_CD8_D0	31	Not Hispanic or Latino	F	Pneumovax day0 CD8p	CD8p	D0	Caucasian	Pneumovax vaccine group	46
GSM744185	009_179_041_CD8_D0	40	Not Hispanic or Latino	M	Flu vaccine day0 CD8p	CD8p	D0	Caucasian	Influenza vaccine group	41
GSM744186	009_179_046_CD8_D1	31	Not Hispanic or Latino	F	Pneumovax day1 CD8p	CD8p	D1	Caucasian	Pneumovax vaccine group	46
GSM744187	009_179_042_CD8_D0	26	Not Hispanic or Latino	M	Flu vaccine day0 CD8p	CD8p	D0	Caucasian	Influenza vaccine group	42
GSM744188	009_179_048_CD8_D0	40	Hispanic	F	Pneumovax day0 CD8p	CD8p	D0	Caucasian	Pneumovax vaccine group	48
GSM744189	009_179_045_CD8_D0	45	Not Hispanic or Latino	M	Pneumovax day0 CD8p	CD8p	D0	Caucasian	Pneumovax vaccine group	45
GSM744190	009_179_043_CD8_D0	36	Not Hispanic or Latino	F	Flu vaccine day0 CD8p	CD8p	D0	Caucasian	Influenza vaccine group	43
GSM744191	009_179_040_CD8_D1	44		F	Flu vaccine day1 CD8p	CD8p	D1	African American	Influenza vaccine group	40
GSM744192	009_179_040_CD8_D0	44		F	Flu vaccine day0 CD8p	CD8p	D0	African American	Influenza vaccine group	40
GSM744193	009_179_043_neutro_D1	36	Not Hispanic or Latino	F	Flu vaccine day1 Neutr	neutrophils	D1	Caucasian	Influenza vaccine group	43
GSM744194	009_179_044_neutro_D1	23	Not Hispanic or Latino	F	Flu vaccine day1 Neutr	neutrophils	D1	Caucasian	Influenza vaccine group	44
GSM744195	009_179_046_neutro_D0	31	Not Hispanic or Latino	F	Pneumovax day0 Neutr	neutrophils	D0	Caucasian	Pneumovax vaccine group	46
GSM744196	009_179_043_neutro_D0	36	Not Hispanic or Latino	F	Flu vaccine day0 Neutr	neutrophils	D0	Caucasian	Influenza vaccine group	43
GSM744197	009_179_047_neutro_D0	23	Not Hispanic or Latino	M	Pneumovax day0 Neutr	neutrophils	D0	Caucasian	Pneumovax vaccine group	47
GSM744198	009_179_047_neutro_D1	23	Not Hispanic or Latino	M	Pneumovax day1 Neutr	neutrophils	D1	Caucasian	Pneumovax vaccine group	47
GSM744199	009_179_041_neutro_D0	40	Not Hispanic or Latino	M	Flu vaccine day0 Neutr	neutrophils	D0	Caucasian	Influenza vaccine group	41
GSM744200	009_179_048_neutro_D0	40	Hispanic	F	Pneumovax day0 Neutr	neutrophils	D0	Caucasian	Pneumovax vaccine group	48
GSM744201	009_179_046_neutro_D1	31	Not Hispanic or Latino	F	Pneumovax day1 Neutr	neutrophils	D1	Caucasian	Pneumovax vaccine group	46
GSM744202	009_179_042_neutro_D1	26	Not Hispanic or Latino	M	Flu vaccine day1 Neutr	neutrophils	D1	Caucasian	Influenza vaccine group	42
GSM744203	009_179_044_neutro_D0	23	Not Hispanic or Latino	F	Flu vaccine day0 Neutr	neutrophils	D0	Caucasian	Influenza vaccine group	44
GSM744204	009_179_042_neutro_D0	26	Not Hispanic or Latino	M	Flu vaccine day0 Neutr	neutrophils	D0	Caucasian	Influenza vaccine group	42
GSM744205	009_179_045_neutro_D0	45	Not Hispanic or Latino	M	Pneumovax day0 Neutr	neutrophils	D0	Caucasian	Pneumovax vaccine group	45
GSM744206	009_179_039_neutro_D1	26	Not Hispanic or Latino	M	Flu vaccine day1 Neutr	neutrophils	D1	Caucasian	Influenza vaccine group	39
GSM744207	009_179_040_neutro_D1	44		F	Flu vaccine day1 Neutr	neutrophils	D1	African American	Influenza vaccine group	40
GSM744208	009_179_048_neutro_D1	40	Hispanic	F	Pneumovax day1 Neutr	neutrophils	D1	Caucasian	Pneumovax vaccine group	48
GSM744209	009_179_040_neutro_D0	44		F	Flu vaccine day0 Neutr	neutrophils	D0	African American	Influenza vaccine group	40
GSM744210	009_179_045_neutro_D1	45	Not Hispanic or Latino	M	Pneumovax day1 Neutr	neutrophils	D1	Caucasian	Pneumovax vaccine group	45
GSM744211	009_179_039_neutro_D0	26	Not Hispanic or Latino	M	Flu vaccine day0 Neutr	neutrophils	D0	Caucasian	Influenza vaccine group	39
GSM744212	009_179_041_neutro_D1	40	Not Hispanic or Latino	M	Flu vaccine day1 Neutr	neutrophils	D1	Caucasian	Influenza vaccine group	41

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